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Vector Systems Online Exam Quiz

Important questions about Vector Systems. Vector Systems MCQ questions with answers. Vector Systems exam questions and answers for students and interviews.

At times, a second enzyme is used while the central portion is removed and the stuffer fragment is placed there. Choose the statement which doesn’t holds for this process.

Options

A : a. It is a method which is used for avoiding the relegation of central portion before the stuffer fragment is attached over there

B : b. In this method, the central portion is cut with another enzyme after being separated from the arms

C : c. The arms are also cut with this second enzyme

D : d. It is not necessary to remove the fragments generated

Physical separation is also used at times. Choose the correct statement with respect to this method.

Options

A : a. Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient

B : b. In sugar density gradient, the molecules are separated on the basis of density

C : c. The central portion is recovered from the gel or the gradient

D : d. Gel is more efficient than the sugar density gradient

Packaging in vitro is also carried out to introduce the lambda DNA into the cells. Choose the incorrect statement in respect to it.

Options

A : a. Packaging in phage coat is a more efficient process than the normal transformation process

B : b. The lambda DNA is incubated with lysate of lambda infected cells in a concatemeric configuration

C : c. Lysate constitutes of lambda proteins needed for phage assembly

D : d. The amount of non-recombinant phage in the background is less

Packaging in vitro is basically carried out by two different strategies. Choose the incorrect statement for it.

Options

A : a. Separate strains with chain-termination mutations in different genes for coat components are used

B : b. Temperature sensitive cI repressor is used

C : c. Due to heat shock packaging proteins are induced

D : d. Packaging is carried out in each strain

Chain-termination mutations in coat proteins genes are carried out. The coat protein coat genes used are __________

Options

A : a. A gene

B : b. D gene

C : c. E and D gene

D : d. D and A gene

Which of the following is a restriction for cloning in bacteriophage Mu?

Options

A : a. It is limited to handling only small number of phage particles

B : b. It relies on whether the DNA is flanked by two mini Mu genes or not

C : c. Recombination between the elements in the second strain is not a restriction

D : d. Fragments of any genomic DNA can be cloned irrespective of the fact whether or not the phage can replicate in it or not

What is the size of the insert that can be accommodated in the head of bacteriophage PI?

Options

A : a. 30-40 kbp

B : b. 80-95 kbp

C : c. 110-115 kbp

D : d. 200-300 kbp

What is the function of the pac site in the bacteriophage PI?

Options

A : a. It is responsible for the initiation of packaging

B : b. It is responsible for the termination of packaging

C : c. It is responsible for the initiation of replication

D : d. It is responsible for the termination of replication

What is the function of loxP sites?

Options

A : a. It is responsible for linearization of DNA in the host bacterium

B : b. It is responsible for circularization of DNA in the host bacterium

C : c. It is responsible for conversion of single stranded DNA into double stranded DNA

D : d. It is responsible for conversion of double stranded DNA into single stranded DNA

Which of the following is not included as a step in cloning in bacteriophage PI?

Options

A : a. The growth of vector is done as a plasmid by using pBR322 origin of replication

B : b. Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated

C : c. Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites

D : d. Incubation is done with mutant lysogen which doesn’t contains head and tail proteins

What happens after packaging is carried out in the phage head?

Options

A : a. A sequence dependent cleavage of DNA is carried out

B : b. A sequence independent cleavage of DNA is carried out

C : c. They are linearized before they are infected into the bacterial cells

D : d. They are circularized before they are infected into the bacterial cells

Circularization must take place in the bacterial cells before the replication starts.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't Say

How many origins of replication are there once the cyclization is carried out by loxP sites?

Options

A : a. 1

B : b. 2

C : c. 3

D : d. 4

Choose the incorrect statement for PAC vectors.

Options

A : a. In these vectors, the phage packaging signals are removed but the two P1 origin of replication persist

B : b. They resemble BAC vectors

C : c. They accept an insert of upto 300 kbp

D : d. Instability is observed

Choose the correct statement in respect to sacB gene?

Options

A : a. The cloning site for PAC lies on the side of sacB gene

B : b. It is responsible for producing an enzyme which is responsible for catalysing sucrose into glucose and fructose

C : c. Expression of sacB in the presence of sucrose is beneficial

D : d. Levan is non-toxic for E. coli

Selection of molecules by lack of inserts on the basis of the sacB gene is known as:

Options

A : a. positive selection

B : b. negative selection

C : c. counter selection

D : d. sacB selection

F (fertility) factor is used in many bacterial systems for different purposes. Choose the incorrect statement with respect to the F factor.

Options

A : a. It was identified in E. coli for catalysing genetic exchange between cells

B : b. Cells having F factor are called as female

C : c. The F factor is transferred from one cell to another via factors present outside

D : d. They are not related to BAC systems

F factor integration into the host chromosome is called as __________

Options

A : a. F’ plasmid

B : b. F factor and host chromosome recombination

C : c. Hfr (High frequency of recombination)

D : d. Recombinant host chromosome

Excision of the F factor can also be done from the host chromosome. Choose the incorrect statement in respect to it.

Options

A : a. This excision is possible in vivo

B : b. Along with the F factor, host chromosome’s DNA sequences are also excited

C : c. The sequences of host chromosome which can be accommodated in the F plasmid are small in size

D : d. These plasmids are termed as F’ plasmids

The low copy number of F plasmid is essential for the stability of these plasmids.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

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