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Simple Cloning Online Exam Quiz

Important questions about Simple Cloning. Simple Cloning MCQ questions with answers. Simple Cloning exam questions and answers for students and interviews.

Transformation efficiency is defined as __________

Options

A : a. ratio of transformed colonies by microgram of sample DNA that is to be inserted

B : b. ratio of transformed colonies by amount of sample DNA that is to be inserted

C : c. ratio of transformed colonies by microgram of plasmid DNA

D : d. ratio of transformed colonies by amount of plasmid DNA

After carrying out the cloning experiment, the cells are plated on agar. Along with agar, it also contains antibiotic resistant genes, X-gal and an inducer of lacZ gene. Which of the following would grow?

Options

A : a. Cells that have taken up plasmid DNA

B : b. Cells that have taken up genomic DNA

C : c. Cells having no insert

D : d. Cells having no insert

Choose the correct statement for adaptors.

Options

A : a. They are blunt ended at both the ends

B : b. They are single stranded at both the ends

C : c. They may be single stranded at one end and other end may be blunt

D : d. They don’t have extra restriction sites within their sequence

If linkers are combined with other features such as a selectable marker, it is called as __________

Options

A : a. cassette

B : b. modified linker

C : c. adaptors

D : d. induced linker

Plasmids are used for carrying out the cloning procedure. Which of the statement is true for plasmids?

Options

A : a. Bacterial plasmids are linear in nature

B : b. They are single stranded

C : c. Insertion of DNA into plasmid allows it to be propagated in host cells and they are known as vectors because of their this property

D : d. They are not capable of replication in bacteria

Which of the following characteristic is not present in a plasmid on a general basis?

Options

A : a. Multiple cloning site (MCS)

B : b. Origin of replication (ori)

C : c. Antibiotic resistance gene

D : d. Beta galactose genes

Bla is a gene, which is incorrect for it?

Options

A : a. It is an antibiotic resistance genes

B : b. Antibiotic acts by blocking the cross-linking of the bacterial cell wall and thus leading to lysing of cells

C : c. It encodes beta lactamase enzyme

D : d. The beta lactam ring is activated

Which of the characteristics is present in lacZ gene?

Options

A : a. It encodes for beta galactosidase enzyme

B : b. Beta galactosidase enzyme is responsible for cleaving monosaccharides into the constituent elements

C : c. It doesn’t cleaves a substrate called as X-gal

D : d. But if X-gal is cleaved, it liberates pink coloured dye

Which of the following orientation is not propagated to later stages?

Options

A : a. Circular plasmids having genomic DNA

B : b. Intramolecular ligation of plasmids

C : c. Linear molecules

D : d. Head to head ligation of two DNA plasmids

Molecules having new combination of sequences that were not present before are called as __________

Options

A : a. intermolecular ligants

B : b. recombinants

C : c. couple

D : d. intramolecular ligants

Insertion of DNA into lacZ gene may lead to disruption of the gene function.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

Which of the following is true regarding taking up of plasmid DNA in the bacterial cells?

Options

A : a. There are more chances of having two plasmids in a single cell

B : b. There are more chances of having a single plasmid in one cell

C : c. Uptake upto two plasmids is possible but not more than that

D : d. Both are taken up with the same efficiency

If genomic DNA has been inserted instead of the plasmid, what will happen?

Options

A : a. It would lead to inactivation of lacZ gene

B : b. The X-gal substrate would be broken down

C : c. The colonies formed are blue in colour

D : d. The lacZ gene would be intact

Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________

Options

A : a. direct PCR

B : b. colony PCR

C : c. quantitative PCR

D : d. in-situ PCR

Generation of recombinants by randomly cloning fragments of total DNA from an organism is called as __________

Options

A : a. genomic library

B : b. screening

C : c. recombination

D : d. shotgun cloning

The phenomenon of alpha complementation is __________

Options

A : a. ? + ? = ?

B : b. ? = ? + ?

C : c. ? = ? + ?

D : d. It is either ? + ? = ? or ? = ? + ?

An operon is defined as __________

Options

A : a. A related set of genes each having different promoters and are present differently

B : b. A set of genes which are present together but are controlled by different promoters

C : c. A set of genes which are present together and are controlled by the same promoter

D : d. A set of genes which are not present together but controlled by the same promoter

Why the whole lacZ gene can’t be present in the plasmid at one time?

Options

A : a. Because the whole lacZ gene can’t be present anywhere

B : b. The whole lac Z gene is very large in size and the plasmid size is small

C : c. The whole lacZ gene is never functional

D : d. Because the plasmid takes is having a restriction site only for taking up a portion of the lacZ gene

Alpha complementation is an indicator of lacZ system. Which of the statement is incorrect for it?

Options

A : a. One portion of the lacZ gene known as minigene is present in the plasmid

B : b. Another portion is present in the host itself

C : c. If they both are allowed to combine in the presence of IPTG, X-gal and ampicillin, blue colonies are observed

D : d. If insert is also present along with host and plasmid, it results in the formation of blue colonies

What will be the consequence of not having an origin of replication (ori) in the vector?

Options

A : a. If an ori is absent, replication of vector would not take place

B : b. As the cells divide after taking up the vector, both the daughter cells would be having the vector

C : c. A colony of transformed colonies is observed

D : d. The vector won’t be taken up by the cell

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