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Polymerase Chain Reaction Online Exam Quiz

Important questions about Polymerase Chain Reaction. Polymerase Chain Reaction MCQ questions with answers. Polymerase Chain Reaction exam questions and answers for students and interviews.

The process of amplification of specific DNA sequences by an enzymatic process is termed as ____________


A : a. amplification

B : b. polymerase chain reaction (PCR)

C : c. translation

D : d. microarrays

What are primers?


A : a. Primers are the short sequences at the end of the nucleotide sequences which are used for amplification

B : b. Primers are the short sequences which are complementary to the nucleotides at the end of the sequence which is to be amplified

C : c. Primers are the short sequences present anywhere in the nucleotide sequence to be amplified

D : d. Primers are the short sequences which are complementary to the nucleotides anywhere in the sequence to be amplified

A reaction mixture for PCR consists of ____________


A : a. heat unstable polymerase

B : b. primers in a limited amount

C : c. deoxynucleoside triphosphate (dNTPs)

D : d. a region complementary to the sequence to be amplified

Which of the following is a characteristic of Taq polymerase?


A : a. It is an RNA polymerase

B : b. It is heat stable

C : c. It is obtained from thermophilic bacterium and can be grown in the laboratory below a temperature of 75 degrees

D : d. It is used in cellular synthesis processes and the optimum temperature is at least 90 degrees

These are steps taken in carrying out the PCR reaction: i) Attaching of primers by cooling ii) Denaturation of strands iii) DNA synthesis iv) Heating Which is the correct order? (Mentioned from starting to ending the reaction)


A : a. i)-ii)-iii)-iv)

B : b. ii)-i)-iii)-iv)

C : c. iv)-iii)-ii)-i)

D : d. iv)-ii)-i)-iii)

Which of the following is not a condition for PCR?


A : a. Initial melting carried out for 5 minutes at 94 degrees

B : b. Initial melting followed by 30 cycles each consisting of melting for 1 minute at 94 degrees

C : c. Renaturation for 5 minutes at 60 degrees

D : d. DNA synthesis at 72 degrees for 1.5 minutes

Primers and polymerases are added again during the reaction because they get consumed as the reaction proceeds.


A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

All the molecules generated during PCR will not be full length. Some will also be of intermediate length. Which of the statements is correct?


A : a. After first cycle, majority of the molecules will be full length and only some will be of intermediate length

B : b. In the next cycle, each intermediate molecule will generate one intermediate molecule and one target molecule

C : c. The number of full length molecules increase as number of cycles proceed

D : d. The number of intermediate molecules increase geometrically and the number of target molecules increase arithmetically

Which of the following activity is not present in Taq polymerase?


A : a. 5’-3’ polymerase

B : b. 5’-3’ exonuclease

C : c. 3’-5’ exonuclease

D : d. Both 5’-3’ polymerase and 5’-3’ exonuclease

What is the half life cycle for Taq polymerase?


A : a. 40 minutes

B : b. 80 minutes

C : c. 10 minutes

D : d. 50 minutes

Taq polymerase incorporates which residue at 3’ end?


A : a. G

B : b. T

C : c. A

D : d. C

Polymerases are also available from other Thermus species. Which of the following is correct?


A : a. Thermus flavus gives Tfl enzyme

B : b. Thermus thermopilus gives Tfl enzyme

C : c. They are having proof reading activity

D : d. Thermus flavus gives Tth enzyme

Polymerases are available with proof reading activity. Which of the following are the characteristics of these types of polymerases?


A : a. They add A residue at 3’ end

B : b. They are obtained from Thermococcus litoralis

C : c. They can’t be obtained from archaebacteria

D : d. The marine bacteria from which they are obtained grow at temperatures lower than that of Thermus aquatics

Thermococcus litoralis grows at a temperature upto 98 degrees.


A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

Primers are generally ____________


A : a. 20-30 nucleotides long

B : b. 40-50 nucleotides long

C : c. as long as the template is

D : d. taken according to the amount available

Which end of the primer should be matched properly in order to carry out the amplification?


A : a. 5’ end

B : b. 3’ end

C : c. Both of the ends should be matched properly

D : d. Anyone of the ends should match

Which of the following nucleotides should be there at 3’ end?


A : a. Any of A, T, G or C will work out

B : b. Either A or T

C : c. Either G or C

D : d. Specifically G

Melting temperature is given by ____________


A : a. 4(G+C) + 2(A+T)

B : b. 2(G+C) + 4(A+T)

C : c. 2(A+G) + 4(C+T)

D : d. 4(A+G) + 2(C+T)

Both the primers, the start primer and the end primer should have a nearly same melting temperature.


A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

Why internal secondary structures are not preferred for primers?


A : a. Internal structures are very bulky and thus elongation is not preferred

B : b. Because of it, primer may fold back on itself and won’t be available for template

C : c. Internal secondary structures require more amount of template

D : d. If internal structures are present, no proof reading would be observed

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