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Modifications and Mutagenesis Online Exam Quiz

Important questions about Modifications and Mutagenesis. Modifications and Mutagenesis MCQ questions with answers. Modifications and Mutagenesis exam questions and answers for students and interviews.

Direct alteration of particular parts of protein as a way of probing the relationship between structure and function is termed as:

Options

A : a. genetic engineering

B : b. protein engineering

C : c. alteration of protein function

D : d. structure engineering

At times, the gene which is cloned is not well known for the protein encoded by it. To access the function, the endogenous gene for the mutant strain is inactivated. This technique is called as ___________

Options

A : a. reverse genetics

B : b. protein engineering

C : c. mutation

D : d. location of function

Approaches for creating mutations can be divided into how many types?

Options

A : a. 1

B : b. 2

C : c. 3

D : d. 4

In the case of deletion of a restriction site, it should be cleaved with the same restriction enzyme.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?

Options

A : a. complete

B : b. partial

C : c. carried out by a different enzyme then that the restriction site

D : d. carried out by a mixture of restriction enzymes

If a functional gene is disrupted while disrupting a restriction site _______ is created.

Options

A : a. frameshift mutation

B : b. point mutation

C : c. either of the mutations

D : d. any other kind of mutation

Additional restriction sites can be introduced near the existing restriction site by mutagenesis.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is _______ and ______ to the original restriction site.

Options

A : a. not only similar, also exactly same

B : b. similar, not same

C : c. not similar, is entirely different

D : d. different, near

Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease.

Options

A : a. double stranded ended

B : b. circular molecules

C : c. single stranded ends

D : d. supercoiled molecules

When a series of deleted regions are replaced by some other DNA fragment of equal length, then it is known as:

Options

A : a. linker scanning

B : b. generation of deletions

C : c. mutation

D : d. replacement

Bisulphite ions are used to deaminate ________ residues in _______ DNA.

Options

A : a. C, double stranded

B : b. C, single stranded

C : c. U, double stranded

D : d. U, single stranded

For mutagenesis without PCR, which of the following can be used as a template?

Options

A : a. Single stranded DNA

B : b. Double stranded DNA

C : c. Circular DNA

D : d. Both single and double stranded DNA

An oligonucleotide is synthesized which contains the mutation and the rest is _______ to the template DNA.

Options

A : a. complementary

B : b. non-complementary

C : c. can either be complementary or non-complementary

D : d. not related

If the template is double stranded, they need to be separated before annealing of oligonucleotide.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

Which of the following statement is incorrect for the synthesis of the second strand?

Options

A : a. The oligonucleotide is acting as a primer for the synthesis of the second strand

B : b. DNA polymerase and dNTPs are added for synthesis

C : c. The polymerase should have 5’-3’ exonuclease activity

D : d. A polymerase having 5’-3’ exonuclease activity would degrade the primer that carries the mutant sequence

Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?

Options

A : a. 1

B : b. 2

C : c. 3

D : d. 4

How many sites can be mutated at a time?

Options

A : a. 1

B : b. 2

C : c. 3

D : d. Many

Several different mutations can be induced at one site.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

If mixed oligonucleotides are used, it is regarded as ______________

Options

A : a. mixed mutagenesis

B : b. multiple mutagenesis

C : c. cassette mutagenesis

D : d. polymutagenesis

It is easier to subclone a restriction fragment if it belongs to?

Options

A : a. small gene

B : b. large gene

C : c. prokaryotic organism

D : d. eukaryotic organism

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