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Immobilized Enzyme Online Exam Quiz

Important questions about Immobilized Enzyme. Immobilized Enzyme MCQ questions with answers. Immobilized Enzyme exam questions and answers for students and interviews.

1. The effectiveness factor increases with the

Options

A : increase of diffusivity and decreased with the increase of particle size

B : increase of diffusivity and increased with the increase of particle size

C : decrease of diffusivity and decreased with the increase of particle size

D : increase of diffusivity and decrease with the decrease of particle size

2. The most commonly employed cross-linked polymer is the

Options

A : polyacrylamide gel

B : collagen

C : celluloses

D : cation exchange resin

3. Which of the following is considered as a disadvantage to the adsorption method of immobilization?

Options

A : It is possible to separate and purify the enzymes while being immobilized

B : The enzymes are not usually deactivated by adsorption

C : The adsorption is a reversible process

D : State of immobilization is very sensitive to solution pH, ionic strength and temperature

4. The immobilized enzyme produced by micro encapsulation technique provides

Options

A : an extremely large surface area

B : smaller surface area

C : high amount of solvent

D : relatively smaller surface area

5. Which of the following is the commonly employed adsorbents?

Options

A : Calcium carbonate

B : Alumina

C : Celluloses

D : All of these

6. Various techniques are available for determining the effective diffusivity of solute in gel. Thin disk method uses

Options

A : a diffusion cell with two compartments divided by a thin gel

B : a diffusion cell with two compartments

C : a diffusion cell with two compartments plus by a thin gel

D : a diffusion cell with two compartments multiply by a thin gel

7. Which medium is used for the production of Penicillin using immobilized cells

Options

A : 1% peptone medium

B : glucose medium

C : Yeast extract medium

D : LB broth

8. Which of the following is not a physical method of immobilization?

Options

A : Adsorption

B : Entrapment

C : Micro encapsulation

D : None of these

9. Damkohler number(N Da ) is

Options

A : the ratio of the maximum reaction rate to the maximum mass transfer rate

B : the ratio of the minimum reaction rate to the maximum mass transfer rate

C : the ratio of the maximum reaction rate to the minimum mass transfer rate

D : the ratio of the minimum reaction rate to the minimum mass transfer rate

10. If Damkohler number, N Da >>1, the reaction rate is much greater than the mass transfer rate and the overall reaction is controlled by the rate of mass transfer. In this case, the enzyme reaction can be described as (where C sb and C s are the substrate concentration in bulk of solution and at the immobilized enzyme surface respectively. k s mass transfer coefficient, and a is the surface area of an immobilized enzyme particles)

Options

A : r p = k s a(C sb -C s )

B : r p = k s aC sb

C : r p = k s aC s

D : r p = k s a(C sb +C s )

1. Which of the following is not the correct answer?

Options

A : Immobilized enzyme may show selectively altered chemical properties

B : Immobilized enzyme may show selectively altered physical properties

C : Immobilized enzyme may simulate the realistic natural environment where the enzyme come from the cell

D : None of the above

2. The immobilization technique involving physical method is

Options

A : covalent bond formation dependent

B : non-covalent bond formation dependent

C : both (a) and (b)

D : ionic bond formation dependent

3. Commonly employed water insoluble supports for the covalent attachment of enzyme include

Options

A : acrylamide based polymers

B : polypeptides

C : dextran

D : all of these

4. For a steady state condition, the change of substrate concentration (dC s /dt) is

Options

A : zero

B : 1

C : >1

D : <1

5. To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, ? can be defined as

Options

A : reaction rate/rate if not slowed by diffusion

B : rate if not slowed by diffusion/ reaction rate

C : actual reaction rate/ rate if not slowed by diffusion

D : rate if not slowed by diffusion/ actual reaction rate

1. Which is not the method for producing immobilized enzymes with multifunctional reagents?

Options

A : Enzymes are adsorbed on the surface active support followed by intermolecular cross linking

B : Functional groups are introduced on the support to react co-valently with enzymes

C : Enzymes are cross linked intermolecularly

D : None of the above

2. The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by

Options

A : copolymerization or microencapsulation

B : crosslinking using multifunctional reagents

C : adsorption

D : all of the above

3. The support material for immobilization of cells of Bacillus subtilis is

Options

A : ion exchange resins

B : gelatin

C : Anthracite

D : agarose and carbodiimide

4. Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have

Options

A : low molecular weight

B : high molecular weight

C : high equivalent weight

D : low reactivity

5. The immobilized technique involving chemical method is

Options

A : covalent bond formation dependent

B : non-covalent bond formation dependent

C : both (a) and (b)

D : ionic bond formation dependent

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