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DNA and Genomic Sequencing Online Exam Quiz

Important questions about DNA and Genomic Sequencing. DNA and Genomic Sequencing MCQ questions with answers. DNA and Genomic Sequencing exam questions and answers for students and interviews.

Which of the following is untrue about DNA sequencing methods?

Options

A : a. Purified fragments of DNA cut from plasmid/phage clones or amplified by polymerase chain reaction (PCR)

B : b. Clones of DNA fragments are denatured to single strands, and one of the strands is hybridized to an oligonucleotide primer

C : c. Taq polymerase is quite heat sensitive

D : d. New strands of DNA are synthesized from the end of the primer

Using ddATP, the resulting synthesis creates a set of nested DNA fragments, each one ending at one of the as in the sequence through the substitution of a fluorescent-labeled ddATP.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

The combined mixture of all labeled DNA fragments is electrophoresed to _____ the fragments by______ and the ladder of fragments is scanned for the presence of each of the four labels.

Options

A : a. separate, size

B : b. separate, pH

C : c. assimilate, pH

D : d. assimilate, size

The sequence can also be verified by making an oligonucleotide primer complementary to the distal part of the readable sequence and using it to obtain the sequence of the complementary strand on the original DNA template.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

When the process is fully automated, a number of priming sites may be used to obtain sequencing results that give optimal separation of bands in each region of the sequence.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

To sequence larger molecules, individual chromosomes are purified and broken into _____ or larger random fragments, which are cloned into vectors designed for large molecules.

Options

A : a. 100-Mb

B : b. 100-kb

C : c. 5000-kb

D : d. 600-kb

Many levels of clone redundancy may be required to build a consensus map because individual clones can have _______

Options

A : a. rearrangements

B : b. deletions

C : c. two separate fragments

D : d. vectors

Once the correct map has been obtained, unique overlapping clones are chosen for sequencing.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

An alternative method is to sequence all the subclones, produce a computer database of the sequences, and then have the computer assemble the sequences from the overlaps that are found.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

Which of the following is untrue about Shotgun Sequencing?

Options

A : a. When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to overlap

B : b. When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to scrutinize

C : c. In a new whole shotgun approach, Celera Genomics is sequencing both ends of DNA fragments of short (2 kb), medium (10 kb), and long (BAC or >100 kb) lengths

D : d. A large number of reads are then assembled by computer

Two common goals in sequence analysis are to identify sequences that encode proteins, which determine all cellular metabolisms, and to discover sequences that regulate the expression of genes or other cellular processes.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

cDNA libraries have been prepared that have the same sequences as the mRNA molecules produced by organisms, or else cDNA copies are sequenced directly by RT-PCR (copying of mRNA by reverse transcriptase followed by sequencing of the cDNA copy by the polymerase chain reaction).

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

Using cDNA sequence with the __________ it is much simpler to locate protein-encoding sequences in these molecules.

Options

A : a. exons taken out

B : b. exons removed

C : c. introns added

D : d. introns removed

An important development for computational purposes was the decision by Craig Venter to prepare databases of partial sequences of the expressed genes, called expressed sequence tags or ESTs.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

The translated sequence can then be compared to a database of protein sequences with the hope of finding a strong similarity to a protein of known function, and hence to identify the function of the cloned EST.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

Investigators are encouraged to submit their newly obtained sequences directly to a member of the International Nucleotide Sequence Database Collaboration, such as the NCBI, DDBJ, and EMBL.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

NCBI reviews new entries and updates existing ones, as requested.

Options

A : a. True

B : b. False

C : c. May be True or False

D : d. Can't say

Which of the given statements is incorrect?

Options

A : a. The simplest and newest way of submitting sequences is through the Web site on a Web form page called BankIt

B : b. The sequence can also be annotated with information about the sequence, such as mRNA start and coding regions

C : c. The submitted form is transformed into GenBank format and returned to the submitter for review before being added to GenBank

D : d. Sequin does not run on UNIX

Which of the given statements is untrue?

Options

A : a. There is no detailed check of sequence accuracy prior to submission to GenBank and other databases

B : b. Often, a sequence is submitted at the time of publication of the sequence in a journal article, providing a certain level of checking by the editorial peer review process

C : c. No sequence is submitted without being published or prior to publication

D : d. In laboratories performing large sequencing projects, such as those engaged in the Human Genome Project or the genome projects of model organisms, the granting agency requires a certain level of accuracy of the order of 1 possible error per 10 kb

Granting agency requires a certain level of accuracy in case of errors. Which of the given statements is untrue regarding it?

Options

A : a. In other laboratories, such as those performing a single-attempt sequencing of ESTs, the error rate may be much higher, approximately 1 in 100, including incorrectly identified bases and inserted or deleted bases

B : b. Incorrect bases always translate to the right amino acid

C : c. Base insertions/deletions will cause frame-shifts in the sequence

D : d. Making alignment with a protein sequence becomes difficult because of frameshifts

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