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Cloning Vectors Online Exam Quiz

Important questions about Cloning Vectors. Cloning Vectors MCQ questions with answers. Cloning Vectors exam questions and answers for students and interviews.

1. Cosmids lack


A : genes coding for viral proteins

B : origin of replication

C : marker genes coding for replication

D : cleavage site for the insertion of foreign DNA

2. A plasmid can be considered as a suitable cloning vector if


A : it can be readily isolated from the cells

B : it possesses a single restriction site for one or more restriction enzymes

C : insertion of foreign DNA does not alter its replication properties

D : All of the above

3. Which of the following is not true about phagemid?


A : Contain functional origin of replication of the plasmid and ? phage

B : May be propagated as a plasmid or as phage in appropriate strain

C : Contain ? att site

D : Can only be propagated as phage

4. Size of the DNA that can be packaged into a ? phage is


A : 50 kb

B : 35-53 kb

C : 40-50 kb

D : any size

5. Inserted DNA in ? gt 11 can be expressed as


A : ?-galactosidase fused protein

B : free protein in the cytoplasm

C : free protein that is secreted out

D : all of the above

6. Cryptic plasmids


A : do not exhibit any phenotypic trait

B : exhibit many phenotypic traits

C : exhibit one phenotypic traits

D : exhibit antibiotic resistance

7. Charon vectors are different from EMBL vectors because


A : they have more extensive range of restriction targets with in their polylinkers.

B : physical separation of lambda arm from central fragment is required

C : both (a) and (b)

D : physical separation of lambda arm from central fragment is not required

8. EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to


A : 6-7 kb

B : 15-25 kb

C : 40-44 kb

D : 1-2 kb

9. Cos site of the cosmids


A : consists of 12 bases

B : helps whole genome in circularization and ligation

C : both (a) and (b)

D : contains cleavage site

10. Maximum size of foreign DNA that can be inserted into an insertion vector is


A : 35 kb

B : 18 kb

C : 50 kb

D : 27 kb

1. Plasmids which are maintained as limited number of copies per cell are known as


A : stringent plasmids

B : relaxed plasmids

C : cryptic plasmids

D : all of these

2. Conjugative plasmids


A : exhibit antibiotic resistance

B : do not exhibit antibiotic resistance

C : carry transfer genes called the tra genes

D : do not carry transfer genes

3. Plasmids which are maintained as multiple copy number per cell are known as


A : stringent plasmids

B : relaxed plasmids

C : cryptic plasmids

D : none of these

4. Maximum size of foreign DNA that can be inserted into a replacement vector is


A : 25-30 kb

B : 18-20 kb

C : 20-25 kb

D : 40-50 kb

5. Select the wrong statement about plasmids?


A : It is extrachromosomal

B : It is double stranded

C : Its replication depends upon host cell

D : It is closed and circular DNA

6. Charon 34 and Charon 35 can clone DNA upto


A : 1-2 kb

B : 6-7 kb

C : 9-20 kb

D : 30-35 kb

7. Cosmid vectors are


A : plasmids that contain fragment of ? DNA including the cos site

B : phages that lack cos site

C : plasmids that have no selection marker

D : cryptic plasmids

8. Single stranded vectors are useful


A : for sequencing of cloned DNA

B : for oligonucleotide directed mutagenesis

C : for probe preparation

D : all of the above

9. Stuffer is


A : the right arm of the vector DNA

B : the left arm of the vector DNA

C : central fragment of the vector DNA

D : none of the above

10. Phagemid consist of


A : plasmid vector carrying ? phage's cos site

B : plasmid vector carrying ? attachment (? att) site

C : plasmid vector carrying origin of replication of ? phage only

D : plasmid vector carrying origin of replication of plasmid only

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