Important questions about Basics of Genetic Engineering. Basics of Genetic Engineering MCQ questions with answers. Basics of Genetic Engineering exam questions and answers for students and interviews.
Nucleic acid precipitated constitutes of ____________
A : a. plasmid DNA
B : b. plasmid DNA, along with RNA and chromosomal DNA
C : c. rna alone
D : d. chromosomal DNA only
Treatment with exonuclease leads to removal of ____________
A : a. remnants of chromosomal DNA
B : b. RNase
C : c. plasmid DNA which is circularized
D : d. proteins
Adsorption onto a solid phase support followed by elution is used as an alternative for separation of which component?
A : a. chromosomal DNA
B : b. plasmid DNA
C : c. RNA alone
D : d. other impurities
Which of the following components bind to the solid column made of silica, under high salt concentration?
A : a. Proteins
B : b. Polysaccharides
C : c. Both proteins and polysaccharides
D : d. Plasmid DNA
Purification of DNA by using silica derivatized groups by DEAE is termed as ____________
A : a. ion exchange resin based method
B : b. silica based purification
C : c. atom based resin exchange method
D : d. packed bed purification
Which of the following is correct with respect to caesium chloride centrifugation?
A : a. Caesium is light in weight
B : b. The dissolution of caesium and nucleic acids leads to the formation of gradients
C : c. According to the amount of supercoiling and A+T content, the DNA settles
D : d. Nicked DNA settles below than supercoiled DNA
Which of the following components settles at the bottom?
A : a. RNA
B : b. Proteins
C : c. Nicked DNA
D : d. Supercoiled DNA
The location of plasmid DNA can be visualized by addition of:
A : a. bromophenol blue
B : b. ethidium bromide
C : c. ortho xylene
D : d. texas red
Which one of the following will travel fastest through the gel if the amount of DNA present is same in all?
A : a. Circular
B : b. Supercoiled
C : c. Nicked
D : d. Nicked
How is the size of molecules under analysis measured?
A : a. By measuring the distance moved through a ruler
B : b. By measuring the amount of visualising dye used
C : c. By running a standard molecule, whose size is known in parallel
D : d. There is no exact criterion for doing so
Gel matrices are generally of two types Agarose and Polyacrylamide. Which of the statements is correct with respect to agarose gels?
A : a. Agarose is a polysaccharide which is obtained from red algae
B : b. Agarose is a polysaccharide obtained from fungus
C : c. It is composed of glucose residues
D : d. It is obtained via dissolving in its water by boiling in water and then cooling it
Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?
A : a. They are obtained via polymerization between acrylamide and bis-acrylamide
B : b. The components added for initiating polymerization are ammonium persulphate and TEMED
C : c. It is casted in horizontal and flat trays
D : d. TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking
If the amount of agarose added is more, the molecular under analysis should have the following characteristic:
A : a. small size
B : b. large size
C : c. size has no relation with the amount of agarose
D : d. the amount of molecules under analysis matters
In the case of electrophoresis of single stranded DNA or RNA, which type of gels are used?
A : a. Renaturing
B : b. Denaturing
C : c. The routine agarose gel
D : d. The routine polyacrylamide gel
At times, a specific fragment of the molecules of DNA which are analyzed needs to be separated. One of the methods is digesting the gels simply. Which of the following statement is not correct in with respect to it?
A : a. The gelling temperature has an important role to play
B : b. For higher gelling temperatures, digestion of DNA is done via the addition of agarase or some chaotropic agents
C : c. For lower gelling temperatures, simply slicing out of target DNA is done which is followed by melting
D : d. The DNA can be extracted via the addition of sodium or any positively charged group
UV shadowing is used at times for visualising the DNA. Which of the statements is correct for it?
A : a. In this method, the DNA bands are visualized because they fluoresce under UV
B : b. The DNA bands won’t fluoresce but will absorb the UV light
C : c. It is not suitable for visualizing single stranded molecules
D : d. It is less preferred over the use of ethidium bromide
The greatest separation is obtained in which portion of the gel?
A : a. Lower portion where the anode is
B : b. Lower portion where the cathode is
C : c. The separation is uniform all over
D : d. It varies according to the quantity of the size of the molecules to be separated
Which of the statements is correct for non uniform separation obtained other than the conventional one?
A : a. It can be obtained via applying strong field towards the cathode
B : b. It can be obtained via applying the same field on both the sides
C : c. The separation is greater on the side of stronger field
D : d. Its is greater towards the weaker field
How can gradient in the field strength be obtained?
A : a. It can be obtained via varying the amount of current, keeping the resistance constant
B : b. It can be obtained via varying the amount of resistance, keeping the current constant
C : c. Both can be varied, but resistance is having more effect
D : d. Buffer-gradient gels can be used with decreasing concentration of buffer at the bottom
If the molecules to be separated are larger than the size for conventional electrophoresis, which type of gels can be used?
A : a. Wedge gels
B : b. Buffer-gradient gels
C : c. Pulse field gels
D : d. Varying the amount of agarose will carry out the separation