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Animal Cell Culture And Regulation Online Exam Quiz

Important questions about Animal Cell Culture And Regulation. Animal Cell Culture And Regulation MCQ questions with answers. Animal Cell Culture And Regulation exam questions and answers for students and interviews.

1. Cells which have undergone transformation frequently become

Options

A : anchorage independent

B : anchorage dependent

C : stable

D : unstable

2. Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

Options

A : Down's syndrome

B : Turner's syndrome

C : Klinefelter's syndrome

D : all of these

3. For culturing, plasma from the adult chicken is preferred to mammalian plasma because

Options

A : it forms a clear and solid coagulum even after dilution

B : it is too opaque

C : it doesn't produce solid clots

D : it forms a semi solid coagulum

4. In animal cell culture, particularly mammalian cell culture, transformation means

Options

A : uptake of new genetic material

B : phenotypic modifications of cells in culture

C : both (a) and (b)

D : release of genetic information

5. Which of the followings are the metabolic products of glucose and glutamine?

Options

A : CO 2 and NH 3

B : CO 2 and lactate

C : Lactate and ammonium

D : Lactate only

6. An established cell line can be called where it has been subcultured at least

Options

A : 70 times at an interval of 3 days between subcultures

B : 40 times at an interval of 3 days between subcultures

C : 70 times at an interval of 1 day between subcultures

D : 50 times at an interval of 3 days between subcultures

7. Which of the following is not the explantation technique?

Options

A : Slide culture

B : Carrel flask culture

C : Roller test tube culture

D : Adherent primary culture

8. Range of osmolarity tolerated/accepted in mOsm/Kg of H 2 O by mammalian cells is

Options

A : 150-300

B : 280-360

C : 300-325

D : 360-400

9. Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

Options

A : 0.05-5 nmol of O 2 /cell/h

B : 5 - 10 nmol of O 2 /cell/h

C : 0.05-5 pmol of O 2 /cell/h

D : 5 - 10 pmol of O 2 /cell/h

10. Range of optimum glucose concentration present in the culture media is

Options

A : 5.5 - 55 mmol/litre

B : 55 - 75 mmol/litre

C : 75-105 mmol/litre

D : 105-150 mmol/litre

1. At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

Options

A : oxidative pathway

B : anaerobic pathway

C : both (a) and (b)

D : none of these

2. Toxicity due to accumulation of ammonia can be overcome

Options

A : by substituting glutamine by glutamate

B : by controlled addition of glutamine at low level

C : by removal of ammonia or ammonium from culture medium

D : all of the above

3. Which of the following is incorrect?

Options

A : Established cell lines (ECL) have short doubling time

B : ECL are invariably aneuploid

C : ECL grow in higher density

D : ECL do not show much evidence of spatial orientation

4. The technique of organ culture may be divided on the basis of employing

Options

A : solid medium

B : liquid medium

C : both (a) and (b)

D : semi-solid medium

5. According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

Options

A : 6 amino acids

B : 8 amino acids

C : 10 amino acids

D : 12 amino acids

6. What is the concentration of CO 2 required for culturing animal cells?

Options

A : 2-5%

B : 1-10%

C : 10-15%

D : 15-20%

7. Accumulation of lactate leads to

Options

A : increase in pH

B : no change in pH

C : reduction in the pH of culture hence loss of cell viability

D : no loss of cell viability

8. Which of the following is the technique used for the embryo culture?

Options

A : Organ cultures on plasma clots

B : Organ cultures on agar

C : Whole embryo cultures

D : All of these

9. To prevent the accumulation of lactate

Options

A : low glutamine concentration is required

B : high glutamine concentration is required

C : low glucose concentration is required

D : high glucose concentration is required

10. How does CO 2 help in the cell metabolism during cell culture?

Options

A : It participates in the de novo synthesis of purines and pyrimidines

B : Helps in the cells respiration

C : For monitoring pH of the culture

D : All of the above

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